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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
12/09/2016 |
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Autoria: |
GREEN, M. J.; THOMPSON, D. A.; MACKENZIE, D. J. |
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Afiliação: |
Margaret J. Green, 1-3 Canadian Food Inspection Agency; Dan A. Thompson; Donald J. MacKenzie. |
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Título: |
Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Plant Disease, St. Paul, v. 83, n. 5, p. 482-485, May 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
A simple and efficient procedure for the extraction of high-quality DNA from phytoplasma-infected woody and herbaceous plants for polymerase chain reaction (PCR) detection is described. This procedure does not require phenol, chloroform, or alcohol for the precipitation of nucleic acids. Herbaceous and woody plant material are extracted in an identical manner with no additional purification or enrichment steps required. The method utilizes commercially available microspin-column matrices, and the extraction of total DNA can be achieved in less than 1 h. The method has been used to successfully purify phytoplasma DNA from whole leaves, leaf petioles and midribs, roots, and dormant wood from a diverse selection of plant material. The phytoplasmas detected by PCR include pear decline, western X-disease, peach yellow leaf roll, peach rosette, apple proliferation, Australian grapevine yellows, and Vaccinium witches'-broom. |
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Palavras-Chave: |
Diagnose; Extração de DNA; Fitoplasma; PCR. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01499naa a2200193 a 4500
001 1840454
005 2016-09-12
008 1999 bl --- 0-- u #d
100 1 $aGREEN, M. J.
245 $aEasy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction.
260 $c1999
520 $aA simple and efficient procedure for the extraction of high-quality DNA from phytoplasma-infected woody and herbaceous plants for polymerase chain reaction (PCR) detection is described. This procedure does not require phenol, chloroform, or alcohol for the precipitation of nucleic acids. Herbaceous and woody plant material are extracted in an identical manner with no additional purification or enrichment steps required. The method utilizes commercially available microspin-column matrices, and the extraction of total DNA can be achieved in less than 1 h. The method has been used to successfully purify phytoplasma DNA from whole leaves, leaf petioles and midribs, roots, and dormant wood from a diverse selection of plant material. The phytoplasmas detected by PCR include pear decline, western X-disease, peach yellow leaf roll, peach rosette, apple proliferation, Australian grapevine yellows, and Vaccinium witches'-broom.
653 $aDiagnose
653 $aExtração de DNA
653 $aFitoplasma
653 $aPCR
700 1 $aTHOMPSON, D. A.
700 1 $aMACKENZIE, D. J.
773 $tPlant Disease, St. Paul$gv. 83, n. 5, p. 482-485, May 1999.
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